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Rabbit Anti-ATG7/APG7 antibody (bs-2432R)
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產品編號 bs-2432R
英文名稱 ATG7/APG7
中文名稱 自噬相關蛋白7抗體
別    名 Apg 7; ATG7; APG7 autophagy 7 like; APG7 autophagy 7-like (S. cerevisiae); APG7 like; APG7, S. cerevisiae, homolog of; APG7L; ATG 7; atg7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7 autophagy related 7 homolog; Atg7l; Autophagy 7, S. cerevisiae, homolog of; Autophagy related protein 7; Autophagy-related 7 (yeast); DKFZp434N0735; GSA 7; GSA7; hAGP7; Ubiquitin activating enzyme E1 like protein; ATG7_HUMAN.   
Specific References  (1)     |     bs-2432R has been referenced in 1 publications.
[IF=2.66] Song, Fuyong, et al. "Involvement of autophagy in tri-ortho-cresyl phosphate-induced delayed neuropathy in hens." Neurochemistry International (2013).  WB ;  
研究領域 腫瘤  免疫學  細胞凋亡  細胞自噬  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, 
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 78kDa
細胞定位 細胞漿 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ATG7:21-120/703 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
產品介紹 This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009].

Function:
Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3.

Subunit:
Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.

Subcellular Location:
Cytoplasm (Probable).

Tissue Specificity:
Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

Similarity:
Belongs to the ATG7 family.

SWISS:
O95352

Gene ID:
10533

Database links:

Entrez Gene: 10533 Human

Entrez Gene: 415961 Chicken

Entrez Gene: 74244 Mouse

Entrez Gene: 312647 Rat

Omim: 608760 Human

SwissProt: Q5ZKY2 Chicken

SwissProt: O95352 Human

SwissProt: Q9D906 Mouse

SwissProt: Q641Y5 Rat

Unigene: 38032 Human

Unigene: 275332 Mouse

Unigene: 162765 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品圖片
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-APG7 (bs-2432R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75 kD
Observed band size: 85 kD
Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APG7 Polyclonal Antibody, Unconjugated(bs-2432R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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