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Rabbit Anti-CD63 antibody (bs-1523R)
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說明書: 50ul  100ul  200ul
20ul/480.00元
50ul/880.00元
100ul/1580.00元
200ul/2480.00元
大包裝/詢價

產品編號 bs-1523R
英文名稱 CD63
中文名稱 黑色素瘤相關抗原抗體
別    名 Lysosomal associated membrane protein 3; CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; granulophysin; LAMP 3; LAMP3; lysosome associated membrane glycoprotein 3; CD 63; CD63; CD63 antigen (melanoma 1 antigen); CD63_HUMAN; gp55; granulophysin; LAMP-3; LIMP; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; NGA; Ocular melanoma-associated antigen; PTLGP40; Tetraspanin-30; Tspan-30; Mast cell antigen AD1; ME491; melanoma 1 antigen; Melanoma associated antigen ME491; Melanoma associated antigen MLA1; MGC72893; MLA 1; MLA1; ocular melanoma associated antigen; OMA81H; Tetraspanin 30; Tspan 30; TSPAN30; Tumor biomarkers; Platelets markers.  
研究領域 腫瘤  心血管  免疫學  細胞類型標志物  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, 
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 26kDa
細胞定位 細胞漿 細胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD63:101-200/238 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
產品介紹 The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins.

Function:
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.

Tissue Specificity:
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Similarity:
Belongs to the tetraspanin (TM4SF) family.

SWISS:
P08962

Gene ID:
967

Database links:

Entrez Gene: 967 Human

Omim: 155740 Human

SwissProt: P08962 Human

Unigene: 445570 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD63為溶酶體膜糖蛋白也是一種細胞表面糖蛋白,可表達在多種細胞的表面和胞漿內,血小板活化后即轉移至膜表面。
CD63—又稱黑色素瘤相關抗原MLA1和整合素形成復合物。是血液血小板活化的標志。與一些腫瘤的進展也有關聯。
產品圖片
Sample:
A431 cell(human) Lysate at 40 ug
HL-60 cell(human) Lysate at 40 ug
MCF-7 cell(human) Lysate at 40 ug
U87mg cell(human)Lysate at 40 ug
Primary: Anti- CD63 (bs-1523R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26kD
Observed band size: 53 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD63 Polyclonal Antibody, Unconjugated(bs-1523R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
cell: A549 cells
Incubation: Avoid light, at 4°C for 60 minutes.
Blank control A549 cells(red line),5X10^5/ml, at 4°C for 60 minutes.
primary antibody: abbit Anti-CD63 antibody (bs-1523R-PE,Blue line) ,1:50, at 4°C for 60 minutes.
Blank control: Hep G2 cells(blue).
Primary Antibody:Rabbit Anti- CD23 antibody(bs-1523R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-1523R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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