|別 名||FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.|
|研究領域||腫瘤 細胞生物 免疫學 發育生物學 神經生物學 信號轉導 干細胞 細胞骨架 腫瘤細胞生物標志物|
|交叉反應||Human, Mouse, Rat, Chicken, Pig, Cow, Goat,|
|產品應用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||53kDa|
|免 疫 原||KLH conjugated synthetic peptide derived from human Vimentin:371-466/466|
|純化方法||affinity purified by Protein A|
|儲 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存條件||Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.|
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Homopolymer assembled from elementary dimers. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B (By similarity).
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
Belongs to the intermediate filament family.
Entrez Gene: 7431 Human
Entrez Gene: 22352 Mouse
Omim: 193060 Human
SwissProt: P08670 Human
SwissProt: P20152 Mouse
Unigene: 455493 Human
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
|Alexa Fluor 350 標記|
|Alexa Fluor 488 標記|
|Alexa Fluor 555 標記|
|Alexa Fluor 594 標記|
|Alexa Fluor 647 標記|