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Rabbit Anti-GLUT4 antibody (bs-0384R)
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產品編號 bs-0384R
英文名稱 GLUT4
中文名稱 葡萄糖轉運蛋白4抗體
別    名 insulin-responsive; Glucose transporter GLUT 4; Glucose Transporter GLUT4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GTR4_HUMAN; kug; SLC 2A4; SLC2A4; solute carrier family 2 (facilitated glucose transporter) member 4; Solute carrier family 2, facilitated glucose transporter member 4.  
研究領域 腫瘤  心血管  免疫學  信號轉導  干細胞  糖尿病  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, 
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 54kDa
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GLUT4:401-509/509 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes.

Function:
Insulin-regulated facilitative glucose transporter.

Subcellular Location:
Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.

Tissue Specificity:
Skeletal and cardiac muscles; brown and white fat.

Post-translational modifications:
Sumoylated.

DISEASE:
Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects.

Similarity:
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

SWISS:
P14672

Gene ID:
6517

Database links:

Entrez Gene: 282359 Cow

Entrez Gene: 6517 Human

Entrez Gene: 20528 Mouse

Entrez Gene: 25139 Rat

Omim: 138190 Human

SwissProt: Q27994 Cow

SwissProt: Q29RP5 Cow

SwissProt: P14672 Human

SwissProt: P14142 Mouse

SwissProt: P19357 Rat

Unigene: 380691 Human

Unigene: 10661 Mouse

Unigene: 1314 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

葡萄糖轉運蛋白-4是一種十分重要的葡萄糖轉運體,與胰島素抵抗和2型糖尿病密切相關.葡萄糖轉運蛋白4在細胞內部和細胞膜之間循環流動.實現對葡萄糖的轉運需要葡萄糖轉運蛋白4自身的轉位和活化.葡萄糖轉運蛋白4與肥胖、腫瘤相關聯.
GLUT-2和GLUT-4蛋白這兩個葡萄糖運載體的研究對于糖尿病的胰島素釋放障礙和胰島素抵抗有重要意義.
產品圖片
Sample:
Heart(Rat) Cell Lysate at 40 ug
Primary: Anti-GLUT4 (bs-0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
muscle (mouse) Lysate at 40 ug
heart (mouse) Lysate at 40 ug
Primary: Anti- beta tubulin(bs-0384R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 54 kD
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti- GLUT4 (bs-0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Lane 4: Muscle (Mouse) Lysate at 40 ug
Lane 5: Muscle (Rat) Lysate at 40 ug
Primary: Anti-GLUT4 (bs-0384R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): K562 (blue).
Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (bs-0384R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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